Hb Ohio	beta142(H20)Ala->Asp

CONTACT		External; central cavity; 2,3-DPG pocket
HEMATOLOGY		Erythrocytosis in the heterozygote (PCV 0.54-0.58 l/l)
ELECTROPHORESIS		Hb X and Hb A can be separated at alkaline pH; Hb X moves faster than Hb A; Hb X moves between Hb A and Hb F at acidic pH
CHROMATOGRAPHY		Hb X was isolated on a DEAE-Sephadex column
STRUCTURE STUDIES		Tryptic digestion; separation of peptides by fingerprinting and cation exchange chromatography; amino acid analysis; sequencing
DNA ANALYSES		Not reported; presumed mutation GCC->GAC at codon 142
FUNCTION STUDIES		Increased oxygen affinity; reduced Bohr effect and cooperativity
STABILITY		Normal
OCCURRENCE		Found in three members of a Caucasian family of Scottish-English ancestry
OTHER INFORMATION		Quantity in the heterozygote 47.7%

REFERENCES
1.		Moo-Penn, W.F., Schneider, R.G., Shih, T-B., Jones, R.T., Govindarajan, S., Govindarajan, P.G., and Patchen, L.C.: Blood, 56:246, 1980.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.