Hb F-Hull	Agamma121(GH4)Glu->Lys

ELECTROPHORESIS		Hb FX moves slower than Hb C
IEF		The same
CHROMATOGRAPHY		Hb FX elutes first from a DEAE-cellulose column
CHAIN SEPARATION		AgammaX = 11.5%; Ggamma = 72.5%; Agamma = 16%
STRUCTURE STUDIES		Tryptic digestion of AE-gammaX chain; separation of peptides by fingerprinting; cation exchange HPLC; amino acid analysis
DNA ANALYSES		A GAA->AAA mutation at codon 121
NOTES		Found in babies of English descent; quantity in heterozygotes 7-9% of total Hb F

REFERENCES
1.		Sacker, L.S., Beale, D., Black, A.J., Huntsman, R.G., Lehmann, H., and Lorkin, P.A.: Br. Med. J., 3:531, 1967.
2.		Ahern, E.J., Ahern, V., Wiltshire, B.G., and Lehmann, H.: Biochim. Biophys. Acta, 303:242, 1973.
3.		Nakatsuji, T., Burnley, M.S., and Huisman, T.H.J.: Blood, 66:803, 1985.

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This material is from the book A Syllabus of Human Hemoglobin Variants (1996) by Titus H.J. Huisman, Marianne F.H. Carver, and Georgi D. Efremov, published by The Sickle Cell Anemia Foundation in Augusta, GA, USA. Copyright © 1996 by Titus H.J. Huisman. All rights reserved. Neither this work nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, microfilming and recording, or by any information storage and retrieval systems, without written permission.